Comparative Genomics, Pangenome, and Phylogenomic Analyses of <i>Brenneria</i> spp., and Delineation of <i>Brenneria izadpanahii</i> sp. nov.

Brenneria species are bacterial plant pathogens mainly affecting woody plants. Association of all members with devastating disorders (e.g., acute oak decline in Iran and United Kingdom) due to adaptation pathogenic behavior response host environmental factors. Some species, including B. goodwinii, salicis, nigrifluens, also show endophytic residence. Here we that novel sp. closely related. Gene-based genome/pangenome-based phylogeny divide the genus into two distinct lineages, clades A The were functionally consistent their common special potential activities as determined via annotation functional domains. Pangenome analysis demonstrated core pathogenicity factors highly conserved, an hrp gene cluster encoding a type III secretion system was found except corticis. An extensive repertoire candidate virulence identified. Comparative genomics indicated cell wall degrading enzymes, metabolites/antibiotics, numerous prophages providing new insights Brenneria−host interactions appropriate targets for further characterization. This work not only documented genetic differentiation but delineates more driven understanding by comparison relevant Pectobacteriaceae thereby substantially enriching extent information available genomic investigations.